Hydrodynamic stress in orbitally shaken bioreactors
نویسندگان
چکیده
منابع مشابه
Hydrodynamic stress in orbitally shaken bioreactors
Materials and methods CHO-DG44 cells were cultivated in suspension in 1-L bottles as described in [1]. To determine conditions under which the shear stress was harmful for the cells, the bottles were orbitally shaken on an ES-X platform (Kühner AG, Birsfelden, Switzerland) at agitation rates from 150 to 200 rpm for 24 h. Control cultures were run in parallel with agitation at 110 rpm. The veloc...
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Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...
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Background Orbitally shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). As a consequence, gas transfer issues are thought to limit the effectivene...
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Increased interest in marine resources has led to increased screening of marine fungi for novel bioactive compounds and considerable effort is being invested in discovering these metabolites. For compound discovery, small-scale cultures are adequate, but agitated bioreactors are desirable for larger-scale production. Calcarisporium sp. KF525 has recently been described to produce calcaride A, a...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2011
ISSN: 1753-6561
DOI: 10.1186/1753-6561-5-s8-p39